Front Clean Culture Facility
Four individual Aquatic Habitat Stand-Alone Systems (Aquatic HabitatsTM AH689). Each stand-alone system consists of a rack with five shelves that has its own sump with mechanical filter tray and biofiltration media, titanium heater, water pump, air pump, mechanical canister filter, activated carbon canister filter, UV sterilizer, heat pump/chiller, in-line pH and conductivity probes, automated dosing system for dosing alkalinity/hardness maintenance solutions, programmable automated water renewal solenoid, interchangeable tanks (3L or 10L), and a programmable controller (for all onboard systems). All systems are connected to an automatic top-off valve and can be set up to automatically renew. Overflow screens with different mesh sizes are available. Ideal for small fish culture and holding.
Back Clean Culture Facility
Freshwater (FW) culture system – One rack with n=2, 8’x4’ recirculating water troughs to maintain water temperatures in glass aquaria (20L or 40L). System is set-up with an in-ground sump, ¾ HP sand filter (for mechanical filtration-backwashed weekly), 2nd sump filled with biofloss for biological filtration, a UV sterilizer, and a heat/chiller pump to control temperature. System recirculates and fills automatically using float valves and automated solenoids connected to clean source waters.
Salt water (SW) or estuarine culture system – One rack with n=2, 8’x4’ recirculating water troughs to maintain water temperatures in glass aquaria (20L or 40L). It is a duplicate of the FW culture system above but connected to estuarine or natural seawater sources (~30ppt).
In either FW or SW system, a variety of glass aquaria may be placed in the water troughs with overflows directed into a separate chute on side of trough. Trough water temperature is separate from culture water.
Experimental Exposure Facility
All exposure systems can be connected to clean fresh, estuarine, or sea water sources.
Two automated electric gear pump diluter systems capable of delivering a user-specified dilution factor to 12 tanks (6 treatments including a control) in 10-20L glass tanks held in a temperature controlled water bath. Toxicant line may be connected to a large source of prepared oil-water mixture (WAF, CEWAF) that may be prepared in a large quantity WAF generator housed in an external shed. This system was designed specifically for all wetted parts to be PTFE or stainless steel and ideal for petroleum product exposures.
Mini diluter system – computer automated system that controls syringe pumps to deliver a user-defined series of dilutions of a toxicant (ideal for effluent samples or a stock toxicant solution) to small glass chambers held in a temperature-controlled water bath. This system is ideal for small organisms requiring low water volumes (less than 1L).
Four sediment diluter systems – computer automated system that delivers at a user-defined frequency, overlaying water renewals to trays of containers holding sediment which are held in a temperature controlled water bath. Ideal for prepared sediment or field-collected sediment samples with sediment dwelling organisms.
Five swimming performance systems:
- 2x Small capacity (5L)
- 1x Medium capacity (10L)
- 1x Large capacity (30L)
- 1x X-Large capacity (90L)
Each system is a stand-alone respirometer tunnel used for physiology, energetics, behavior, biomechanics and kinematics of swimming fish. All are computer controlled flow chambers that control flow velocity and record temperature and O2 parameters. Remote viewing provided by cameras and HD monitors.
Adult fish studies — One 40 gal tank (24” x 51” x 8”) that fits in a larger trough to regulate and maintain temperature. Camera mounted above chamber for tracking organisms. Observation tank surrounded by black-out curtain.
Larval fish studies — DanioVision, self-contained exposure and tracking chamber designed to hold well-plates. Ideal for small organisms or embryonic or larval fish.
EthoVisionXT (Noldus) – Video tracking software connected to both systems designed to tracks and analyzes the behavior, movement, and activity of any animal. A cost-effective solution for all standard behavioral tests such as the Morris water maze and open field testing. High-throughput and high-content testing. Suitable for sophisticated test-protocols.
- Zeiss Stemi 2000-C Stereo Microscope with an AxioCam ERc5s camera (4 each)
- Zeiss Standard 25 Light Microscope (5x, 10x, 40x, 100x objectives)
- Olympus Inverted Fluorescence microscope (IX71 Series)
- Thermo Scientific accuSpin Micro 17R Refrigerated Microcentrifuge with 24-tube fixed rotor
- Thermo Scientific Sorvall Legend RT Plus Centrifuge with various size rotors
- New Brunswick Innova 44 Incubator Shaker
- Beckman Coulter Multisizer 4 Particle Counter
- Ohaus Standard Balance (Explorer E1F110)
- Mettler Toledo Analytical Balance (AG135)
- YSI meters: DO (4 each) and salinity–conductivity–temperature (5 each)
- pH meters (6 each)
- Light boxes for organism observations (2 each)
- Stir plates (one with light, three that are also heat plates) (9 each)
- Fume hood
- Bechtop stations, desks, and computers